Table 1.
Gradient elution program to elute (method 1) FOS and inulin and (method 2) scFOS.
| HPAEC-PED method 1 | HPAEC-PED method 2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Elution time (min) | A(%) | B(%) | C1(%) | Comment | Elution time (min) | A(%) | B(%) | C2(%) | Comment |
| −40a | 0 | 100 | 0 | Start cleaning step | −45a | 0 | 100 | 0 | Start cleaning step |
| −30a | 0 | 100 | 0 | End cleaning step | −30a | 0 | 100 | 0 | End cleaning step |
| −29.9a | 89 | 10 | 1 | Start conditioning step | −29.9a | 89 | 10 | 1 | Start conditioning step |
| 0 | 89 | 10 | 1 | End conditioning step | 0 | 89 | 10 | 1 | End conditioning step |
| 0.1 | 89 | 10 | 1 | Injection, acquisition start | 0.1 | 89 | 10 | 1 | Injection, acquisition start |
| 4 | 84 | 15 | 1 | End first gradient step | 5 | 89 | 10 | 1 | End isocratic elution |
| 45 | 60 | 20 | 20 | End first gradient step | |||||
| 80 | 79 | 15 | 40 | End third gradient step | 55 | 50 | 20 | 30 | End second gradient step |
A: deionized water; B: sodium hydroxide (600 mM); C1: sodium nitrate (250 mM); C2: sodium acetate (500 mM).
aNegative time indicates time prior to sample injection.