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Gradient elution program toelute oligosaccharide fraction present in inulin added to cooked ham.
| Elution time (min) | A(%) | B(%) | C1(%) | Comment |
|---|---|---|---|---|
| −35a | 0 | 100 | 0 | Start cleaning step |
| −25a | 0 | 100 | 0 | End cleaning step |
| −24.9a | 79 | 16 | 5 | Start conditioning step |
| 0 | 79 | 16 | 5 | End conditioning step |
| 0.1 | 74 | 16 | 10 | Injection, acquisition start |
| 20 | 62 | 16 | 22 | End first gradient step |
A: deionized water; B: sodium hydroxide (600 mM); C: sodium acetate (500 mM).
aNegative time indicates time prior to sample injection.
