Table 4.
Changes in denaturation temperatures for Pfdpd and Zn-Pfdpd in the presence of metal ions at pH 7.8.
| (mM) | Phdpd | Zn-Phdpd | ||
|---|---|---|---|---|
| T m (°C) | ΔT m | T m (°C) | ΔT m | |
| Zn | ||||
| 0.05 | 118.4 | 16.9 | ||
| 0.02 | 111.6 | 7.2 | 118.4 | 16.9 |
| 0.01 | 110.9 | 6.5 | 118.4 | 16.9 |
|
| ||||
| Co | ||||
| 0.05 | 110.7 | 6.3 | 116.5 | 15.0 |
| 0.02 | 110.3 | 5.9 | 114.7 | 13.2 |
| 0.01 | 109.9 | 5.5 | 112.9 | 11.4 |
|
| ||||
| Mn | ||||
| 0.05 | 110.1 | 5.7 | 114.5 | 13.0 |
| 0.02 | 109.7 | 5.3 | 112.2 | 10.7 |
| 0.01 | 109 | 4.6 | 109.8 | 8.3 |
The denaturation temperatures (T m) were obtained from the peak temperatures of DSC curves. The T m values of Pfdpd and Zn-Pfdpd in the absence of metals were 104.4 and 101.5°C, respectively. All protein concentrations were 0.01 mM.