Mice were subjected to mechanical ventilation at high tidal volume (HTV, 30 ml/kg, 4 hr) with or without TRAP6 injection (3×10−7 mol/mouse i/t), or left spontaneously ventilated. Animals were treated with iloprost (2 μg/kg, i/v) or sterile saline at three time points (0, 40, and 80 min) during mechanical ventilation. Cell count (A) and measurements of protein concentration (B) were performed in BAL fluid taken from control and experimental animals; n=6–10 per condition; *p<0.03, **p<0.005.