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. Author manuscript; available in PMC: 2011 Jan 1.

Published in final edited form as: Transl Res. 2010 Jan;155(1):44. doi: 10.1016/j.trsl.2009.09.002

Pulmonary EC grown to confluence on Flexcell plates were pre-treated with iloprost (200 ng/ml, 15 min) and exposed to 18% CS for indicated time periods. Phosphorylation of MLC (A) and MYPT (B) was detected by western blot with phospho-specific antibodies. HPAEC were further stimulated with thrombin (0.1 U/ml, 15 or 30 min) with or without iloprost (200 ng/ml, 15 min) pretreatment. Phosphorylated MLC (C) and MYPT (D) were detected by immunoblotting with specific antibodies. Results are representative of three to six independent experiments.

Pulmonary EC grown to confluence on Flexcell plates were pre-treated with iloprost (200 ng/ml, 15 min) and exposed to 18% CS for indicated time periods. Phosphorylation of MLC (A) and MYPT (B) was detected by western blot with phospho-specific antibodies. HPAEC were further stimulated with thrombin (0.1 U/ml, 15 or 30 min) with or without iloprost (200 ng/ml, 15 min) pretreatment. Phosphorylated MLC (C) and MYPT (D) were detected by immunoblotting with specific antibodies. Results are representative of three to six independent experiments.