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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Biochemistry. 2010 Jan 26;49(3):635. doi: 10.1021/bi901779p

FIGURE 8. Lys53 and Lys125 of the CI-MPR are required for the receptor’s interaction with Glu-plasminogen.

FIGURE 8

Aliquots containing 10, 25, 50, 75, 100, 150, and 250 nM bovine Glu-plasminogen in HBA buffer containing 150 mM sodium acetate, pH 7.4 were injected onto CM5 sensor chips containing coupled Dom1-3His (μ), K53A (▼;), or K125A (λ). The response at equilibrium (Req) was determined from the resulting sensorgrams and plotted against the concentration of Glu-plasminogen ([Plg]). The data were fit by non-linear regression using a two-site saturation binding model.