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. Author manuscript; available in PMC: 2010 Jan 31.
Published in final edited form as: Methods Mol Biol. 2008;468:157. doi: 10.1007/978-1-59745-249-6_12

Figure 1. Confocal Microscopy for PKC Activation.

Figure 1

Using a GFP-tagged PKCĪ²II expression vector, cells were treated with either control siRNA or siRNA against Wnt5A. In cells co-transfected with a control siRNA (single arrow), PKC (double arrows) remains at its active site in the membrane (A). When these cells are cotransfected with siRNA against Wnt5A (single arrow), PKC (double arrows) moves into the cytoplasm (B).