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. Author manuscript; available in PMC: 2010 Jan 31.
Published in final edited form as: Methods Mol Biol. 2008;468:157. doi: 10.1007/978-1-59745-249-6_12

Figure 3. Concentration and time course determination for rWnt5A treatment.

Figure 3

Cells were assayed for their ability to increase PO4-PKC in response to rWnt5A treatment, at differing concentrations, and over differing times. 16–24 hours was ultimately determined to be the ideal time-course for Wnt5A treatment. Note that at 4 hours Wnt5A effects are consistently dampened, perhaps due to receptor recycling.