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. Author manuscript; available in PMC: 2010 Jan 31.
Published in final edited form as: Methods Mol Biol. 2008;468:157. doi: 10.1007/978-1-59745-249-6_12

Figure 5. Wnt5A is glycosylated in melanoma cells.

Figure 5

UACC647 melanoma cells have high levels of Wnt5A and are highly metastatic. To determine if the Wnt5A in these cells was glycosylated, protein lysates were subjected to treatment with PNGaseF, a de-glycosylase. Post- treatment with this enzyme, Wnt5A migrated at around 38–40kDa as compared to its migration at 42–44kDa pre-treatment. Recombinant Wnt5A was also run as control, and demonstrated the same de-glycosylation.