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. Author manuscript; available in PMC: 2010 May 25.
Published in final edited form as: Chem Biol. 2009 Nov 25;16(11):1169. doi: 10.1016/j.chembiol.2009.10.009

Table 2. Contributions of individual mutations to red-shifting in dNeptune.

Reversion from Neptune Excitation peaka Emission peaka εb φc
none (Neptune) 600 650 72000 0.18
C61S 597 649 80000 0.18
F197Y 596 649 56000 0.16
G41M 595 637 60000 0.21
C158A 593 644 97000 0.19
C61S F197Y G41M C158A (mKate S158A) 585 630 75000 0.30
C61S F197Y G41M (mKate S158C) 586 630 63000 0.33
a

Excitation and emission maxima in nm.

b

Maximum extinction coefficient in M-1cm-1 measured by the alkali denaturation method (Chalfie and Kain, 2005; Gross et al., 2000).

c

Quantum yield of fluorescence.