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| Protein | mNeptune | IFP1.4 |
|---|---|---|
| Peak excitation (nm) | 600 | 684 |
| Peak emission (nm) | 650 | 708 |
| Extinction coefficient (M-1cm-1) | 67000 | 92000 |
| Quantum yield | 0.20 | 0.07 |
| Overall brightness* | 13.4 | 6.4 |
| Photostability* | 160 | 8.4 |
| Green emission component | present | absent |
| Cofactor dependence | none | biliverdin |
| Molecular weight (kD) | 30 | 36 |
| Stoichiometry (at 5 μM) | monomer-dimer equilibrium | monomer |
Calculated as in Table 1.
