Fig. 3.

Anti-oxidant activities of herbs used in Korean traditional medicine assessed by inhibition of fluorescence emitting from DCHF/Fenton reagent reaction. The anti-oxidant activities of 70 herbs were assessed using oxidation of DCHF by Fenton reagent. DCHF (50 µM) was mixed with 60 mM H₂O₂, 0.75 mM FeCl₂ and PBS, pH 7.4 in the absence or presence of each water extract (5 µl) in 96 well plates. Total volume was 200 µl and reaction was started by adding 60 mM H₂O₂ last, allowed at 37℃ for 10 min and then fluorescence was measured. Of the 70 herbs, 59 were shown to inhibit the fluorescence. The anti-oxidant activity was expressed by % inhibition of the control fluorescence [(control fluorescence-experimental fluorescence)/control fluorescence×100]. Numbers given at each herb are the serial numbers shown in Table 1.