Table 1.

Comparison of colocalization analysis methods for simulated images with high SNR in Fig. 1, A and B

	PPI A to B	PPI B to A	Correlation
Real value	0.20	0.71	0.38
Pearson's coefficient	N/A	N/A	0.73
Overlap coefficient	0.13	5.11	0.83
Manders' coefficient	0.98	1.00	N/A
Costes' approach	1.00	0.96	N/A
ICCS (image scrambled)	0.56	0.94	N/A
This article	0.22	0.75	0.41