Table 3.

Comparison of colocalization analysis methods on images of ryanodine receptor (Fig. 4A) and estrogen receptor α (Fig. 4B)

	PPI A to B	PPI B to A	Correlation
Pearson's coefficient	N/A	N/A	0.35
Overlap coefficient	0.15	1.66	0.50
Manders' coefficient	0.81	0.81	N/A
Costes' approach	0.59	0.49	N/A
ICCS (image scrambled)	0.32	0.37	N/A
This article	0.08	0.06	0.07