Table 1.
Kinetic parameters (time constants and relative inputs of components) of subunit ɛ conformational transitions induced by addition of ATP to 1 mM at 59°C
| Sample | τ1, s | A1, % | τ2, s | A2, % | n |
|---|---|---|---|---|---|
| WT F1 | 1.3 ± 0.3 | 58 ± 8 | 9.6 ± 2.4 | 42 ± 8 | 9 |
| WT FOF1 | 7.8 ± 2.6 | 61 ± 4 | 57 ± 22 | 39 ± 4 | 6 |
| βD390A F1 | 1.2 ± 0.4 | 71 ± 8 | 8.0 ± 2.9 | 29 ± 8 | 7 |
| βD390A FOF1 | 3.0 ± 1.4 | 64 ± 6 | 18 ± 4 | 36 ± 6 | 4 |
| βAALSAAA F1 | 0.6 ± 0.3 | 84 ± 4 | 5.7 ± 1.6 | 16 ± 4 | 7 |
| βAALSAAA FOF1 | 0.9 ± 0.2 | 80 ± 2 | 17 ± 3 | 20 ± 2 | 3 |
| ɛEARA FOF1 | 1.8 ± 0.8 | 60 ± 2 | 28 ± 2 | 40 ± 2 | 4 |
Traces were fitted with a biexponential function; n is the number of traces analyzed.