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Cholesterol inhibition of hemolysis by pneumolysin. The hemolytic activity of pneumolysin (PLY) was inhibited by cholesterol. Different concentrations of pneumolysin, or pneumolysin mixed with 1% cholesterol, were incubated with rabbit erythrocytes. Lysis was measured by spectrophotometric analysis of supernatants after incubation and was compared with a saponin control that represented 100% lysis. Data are mean ± SD.
