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The fluorescent images showing capping of SykEGFP at the plasma membrane. SykEGFP was redistributed to the plasma membrane after stimulation with anti-IgM antibody at room temperature for 5 min (a), 30 min (b), 60 min (c) and 90 min (d). The cells labeled by red asterisks in (d) were considered the ones with capping. The percentage of cells with capping was 81±3% (enumerated in three separate experiments by one observer with the results of 396/470, 462/575, and 388/497).
