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Representative results of fluorescent PCR analysis of a pool of wild-type embryos (a) and a pool of embryos from a founder (b). The PCR products from the wild-type embryos sometimes contain more than one peak due to PCR stutter or non-specific amplification. However, the additional peak (b, double asterisks) that is not present in the wild-type control sample indicates the presence of ZFN-induced indel mutation.
