| Component | Amount | Final |
|---|---|---|
| PmeI-linearized ZFN plasmid DNA | 3 μl | 0.5 μg |
| 2x NTP/ARCA | 5 μl | 1x |
| 10x T7 Reaction Buffer | 1 μl | 1x |
| T7 Enzyme Mix | 1 μl |
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