| Component | Amount | Final |
|---|---|---|
| Transcribed RNA (from Step 17) | 11 μl | unknown |
| 5x E-PAP | 10 μl | 1x |
| 25 mM ATP | 5 μl | 2.5 mM |
| 25 mM MnCl2 | 5 μl | 2.5 mM |
| Nuclease-free water | to 50 μl |
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