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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Protoc. 2009;4(12):1855. doi: 10.1038/nprot.2009.209
Component Amount Amount
ZFN-encoding RNA (for the left half site) variable 750 ng
ZFN-encoding RNA (for the right half site) variable 750 ng
1% phenol red 0.5 μl 0.08%
1x Danieau solution to 6 μl