Component	Amount	Final
Genomic DNA (from Step 30)	2.5 μl	unknown
10x High Fidelity PCR Buffer	5 μl	1x
50 mM MgSO4	2 μl	2 mM
10 mM dNTPs	1 μl	0.2 mM
DMSO	2.5 μl	5%
Forward primer (10 μM)	5 μl	1 μM
Reverse primer (10 μM)	5 μl	1 μM
Platinum Taq High Fidelity	0.2 μl
Nuclease-free water	to 50 μl