| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 95 °C, 5 minutes | ||
| 2–25 | 95 °C, 30 seconds | 65 °C, 30 seconds | 68 °C, 45 seconds |
| 26 | 68 °C, 5 minutes |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.