Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Protoc. 2009;4(12):1855. doi: 10.1038/nprot.2009.209
Component Amount Final
Genomic DNA 2.5 μl unknown
10x Taq DNA polymerase buffer 2 μl 1x
10 mM dNTPs 0.4 μl 0.2 mM
Forward primer (10 μM) 1 μl 0.5 μM
Reverse primer (10 μM) 1 μl 0.5 μM
Taq DNA polymerase (5 U/μl) 0.2 μl
Nuclease-free water to 20 μl