| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 95 °C, 5 minutes | ||
| 2–41 | 95 °C, 30 seconds | 65 °C, 30 seconds | 68 °C, 45 seconds |
| 42 | 68 °C, 5 minutes |
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