| Component | Amount | Final |
|---|---|---|
| DpnI/XbaI digested DNA (from Step 5) | 35 μl | unknown |
| 10x NEBuffer BamHI (see REAGENT SETUP) | 4.5 μl | 1x |
| 10x BSA (1 mg/ml) | 1 μl | 1x |
| BamHI (20 U/μl) | 1 μl | 20 U |
| Nuclease-free water | to 45 μl |
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