| Component | Amount | Final |
|---|---|---|
| pMLM 290 or pMLM 292 plasmid DNA | 1 μg | 0.04 μg/μl |
| 10x NEBuffer 2 | 2.5 μl | 1x |
| 10x BSA (1 mg/ml) | 2.5 μl | 1x |
| XbaI (20 U/μl) | 1 μl | 20 U |
| Nuclease-free water | to 25 μl |
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