| Component | Amount | Final |
|---|---|---|
| XbaI digested DNA (from Step 8) | 25 μl | 1 μg |
| 10x NEBuffer BamHI (see REAGENT SETUP) | 3.5 μl | 1x |
| 10x BSA (1 mg/ml) | 1 μl | 1x |
| BamHI (20 U/μl) | 1 μl | 20 U |
| Nuclease-free water | to 35 μl |
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