| Component | Amount | Final |
|---|---|---|
| triple-digested PCR product (from Step 7) | 9 μl | unknown |
| XbaI/BamHI-digested pMLM290 or pMLM292 (from Step 10) | 1 μl | unknown |
| 2x Quick Ligation Reaction Buffer | 10 μl | |
| T4 DNA ligase (400 U/μl) | 1 μl |
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