Figure 6.
FAS contributes to the up-regulation of 5-LOX in a positive feedback loop manner. HepG2-XΔ127 (or H7402-XΔ127) cells (2 x 105) were incubated with or without cerulenin (2.5, 5, and 10 µg/ml) for 12 hours. (A) The mRNA level of 5-LOX was detected by real-time PCR. Plotted are the means ± SD of three samples normalized to β-actin. Statistically significant differences relative to 5-LOX mRNA levels in HepG2-XΔ127 (or H7402-XΔ127) cells without the action of cerulenin, arbitrarily set to 1.0, are indicated: *P < .05, **P < .01, ***P < .001 (Student's t test). (B) The protein expression level of 5-LOX was examined by Western blot analysis. (C, D) The amount of LTB4 was examined by ELISA in the conditioned medium or in the cell lysates from the previously mentioned treated HepG2-XΔ127 (or H7402-XΔ127) cells. Data are representative of three independent experiments. Values represent mean ±S D (n = 3). *P < .05 versus control, **P < .01 versus control, ***P < .001 versus control (Student's t test).