Figure 5.

Decreased IPSC amplitude in NL2 KO mice is accompanied by an increase in the CV and also represents a uniform shift in distribution. A, Representative presynaptic APs evoked in an FS neuron (top, 4 pulses, 20 Hz) and the resulting average postsynaptic unitary IPSCs (middle) in an excitatory neuron. Hollow bar indicates the time window in which individual responses for IPSC1 are plotted (bottom) (for all recordings, APs in current-clamp and 50 mm Cl⁻ in the pipette). The ACSF contained 20 μm DNQX and 100 μm APV. B–D, Plots of the mean IPSC1 amplitude (B), connection frequency (χ² test) (C), and mean CV (B–D) as a function of genotype. E, F, Cumulative distributions for the absolute (E) and scaled (F) unitary IPSC1 amplitudes. E, Distributions are different (p < 0.002, K-S test). F, The NL2[+/+,+/−] distribution was scaled down to 52%: a best fit to the NL2 KO distribution based on the least error sum of squares. After scaling, the distributions are not detectably different (K-S test). All data shown are means ± SEMs (*p < 0.05; **p < 0.01. Student's t test). The genotypic group NL2[+/+,+/−] includes both homozygous and heterozygous mice.