Skip to main content
. 2009 Sep 15;17(12):2121–2130. doi: 10.1038/mt.2009.217

Figure 1.

Figure 1

Genomes and anticancer activity of Ad-GL and Ad-GL-HB in vitro. (a) Open arrows, early Ad regions; closed arrows, late Ad regions; gray arrows, inserted or modified genes; closed boxes, ITRs. Ad-GL and Ad-GL-HB are replication-competent Ad vectors that overexpress ADP (E3-11.6K) for increased spread and express EGFP-firefly luciferase fusion protein for imaging virus transduction, spread, and persistence. Ad-GL-HB has BAP inserted into HVR5 loop of hexon to prevent binding to blood FX and reduce hepatocyte transduction. (b) MDA-MB-468, Hep3B, and A549 cells were infected with Ad-GL or Ad-GL-HB at the indicated multiplicities of infection (MOIs) (n = 4). Five days later EGFP expression (upper row) and cell viability (bottom row) were measured. Data are presented as mean ± SEM. Ad, adenovirus; ADP, adenovirus death protein; BAP, biotin acceptor peptide; EGFP, enhanced green fluorescent protein; FX, factor X; HVR5, hypervariable loop 5; ITR, inverted terminal repeat.