Figure 4.

Increased safety of Ad-GL-HB. (a) Survival of mice injected i.v. with high virus dose. C57BL/6 mice were injected i.v. with 3 × 10¹¹ vp of Ad-GL or Ad-GL-HB (n = 4) on day 0. Mice were monitored till day 16. (b) Hepatotoxicity of Ad-GL and Ad-GL-HB after systemic administration. Blood was collected from survived mice from a and from additional C57BL/6 mice injected with 3 × 10¹⁰ vp of Ad-GL, pretreated or not pretreated with warfarin (n = 4), on day 3 after injection and was analyzed for serum ALT levels as a marker of liver damage. Ad, adenovirus; ALT, alanine aminotransferase; i.v., intravenous; vp, virus particles.