Figure 6.

Multiple high-dose injections of Ad-GL-HB demonstrate improved systemic anticancer activity. (a) Mice with established s.c. DU145 tumors (16 days after cell injection; average tumor volume 337 mm³) were predosed i.v. once with 3 × 10¹⁰ vp of Ad-GL-HB on day 0 followed by one, two, or three daily i.v. injections of 3 × 10¹⁰ vp of Ad-GL-HB (n = 9 or 10). Mice were euthanized when tumor volume reached 2,000 mm³. Kaplan–Meyer survival curves were plotted. (b) Additional mice with s.c. DU145 tumors established as described in a were injected i.v. with 1 × 10¹¹ vp of Ad-GL-HB one or three times weekly with prior predosing with 3 × 10¹⁰ vp of Ad-GL-HB. Mice were euthanized when tumor volume reached 2,000 mm³. Kaplan–Meyer survival curves were plotted. Survival data for the same group of buffer-treated animals is shown in a and b. HD, high dose; i.v., intravenous; LD, low dose; s.c., subcutaneous; vp, virus particles.