Figure 7.

Effect of hexon modification, predosing, and multiple virus injections on IL-6 and ALT levels. (a) C57BL/6 mice were injected i.v. via tail vein with indicated doses of Ad-GL or Ad-GL-HB on day 0 (n = 4). For predosing, mice were injected with 3 × 10¹⁰ vp of Ad-GL-HB 4 hours before dosing. Three hours after the dosing blood was collected and serum levels of IL-6 were measured. (b) Mice from a injected with 3 × 10¹⁰ vp of Ad-GL-HB received additional two i.v. doses of 3 × 10¹⁰ vp of Ad-GL-HB on days 1 and 2. Three days after the last virus injection (day 5 after first injection) blood was collected and serum ALT levels were measured. Mice injected with Ad-GL died on day 3 after the injection. (c) Mice from b were euthanized after blood collection (day 5 after first virus injection) and livers and spleens were collected. Virus genome levels were quantified in the organs by real-time PCR. ALT, alanine aminotransferase; IL, interleukin; i.v., intravenous; vp, virus particles.