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Perfusion index in the ischemic limb. (a) Representative selected zone of major vessels of the leg (included popliteal and tibial vessels, from the knee to the foot) (b) Calculation of perfusion index of the foot. This criterion was determined by pixel analysis and expressed as the ratio of the length of popliteal and tibial vessels in ischemic versus nonischemic limb (R/L ratio). The data are the mean ± SEM (n = 6 animals per group) (*P < 0.05, **P < 0.01 versus control group).
