Skip to main content
. 2009 Oct 6;17(12):2058–2066. doi: 10.1038/mt.2009.225

Figure 4.

Figure 4

In vivo effects of controlled expression of a suicide gene in the normal mouse brain. (a) GFAP immunostaining of brain sections from BV-HSVtk-ctrl- and BV-HSVtk-mix9F-injected mice show the protection of GFAP+ astrocytes by incorporating microRNA targeting sequences into baculoviral expression cassette. The enlarged images of region I and II are shown on the right side. (b) Western blot analysis of GFAP expression in the BV-HSVtk-ctrl- and BV-HSVtk-mix9F-injected mouse brain show incorporation of miRNA targeting sequences prevents reduction in protein content of GFAP. BV, baculoviral vector; Cg, the cingulate cortex; GCV, ganciclovir; GFAP, glial fibrillary acidic protein; HSVtk, the herpes simplex virus thymidine kinase gene; LV, the lateral ventricle; STR, the striatum.