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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2008 Sep;7(9):2798. doi: 10.1158/1535-7163.MCT-08-0276

Figure 1.

Figure 1

siRNA to hKSR-2 selectively reduces the levels of the cognate mRNA and protein. A, HL60-G cells were transfected with either control siRNA (Co-siRNA), siKSR1, or si-hKSR-2 (all 5 μmol/L), incubated for 48 h, and treated with 10 nmol/L 1,25-(OH)2D3 for another 24 h. mRNA levels of KSR1 and hKSR-2 were determined by semiquantitative reverse transcription-PCR. Note that both KSR1 and si-hKSR-2 mRNA levels were induced by 1,25-(OH)2D3 treatment, and siKSR-1 or si-hKSR-2 selectively reduced the cognate mRNA expression. β-Actin RNA was used as a housekeeping gene control. The ratios of absorbance of each gene to β-actin are shown below each lane. B, protein levels of hKSR-2 in cells transfected with si-hKSR-2. Crk-L was used as a loading control. The experiments were repeated three times with similar results.