FIG 6.

Immunofluorescent localization of B. cenocepacia AU0728 and phage BcepIL02 in mouse lungs. C57BL/6 mice were infected with B. cenocepacia AU0728 and treated 24 h later by i.n. inhalation (A, B) or i.p. injection (C, D) of BcepIL02. Lungs were removed 48 h later, fixed, sectioned, and stained for phage (A-D; green) and bacteria (B, C, D; red). Phage administered via i.n. inhalation primarily localized in alveolar macrophages (A, B). B, Arrows indicate localization of degraded bacteria inside macrophages. Phage administered via i.p. injection were found primarily in perivascular areas (C) and alveolar septa, where they could be observed co-localized with degraded bacteria (D). (A, bar = 50 μm; B, C, bar = 20 μm; D, bar = 10 μm).