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. 2010 Feb;129(2):178–185. doi: 10.1111/j.1365-2567.2009.03174.x

Figure 3.

Figure 3

Expression of inflammasome components in rheumatoid arthritis (RA) synovial fibroblasts. (a) Expression of NACHT, LRR and PYD domains containing proteins (NALPs) 1–14, apoptosis-associated speck-like protein containing a CARD domain (ASC), caspase 1 and caspase 5 messenger RNA assessed by reverse transcription–polymerase chain reaction and two of their substrates interleukin-1β (IL-1β) and IL-18 in RA fibroblast-like synoviocytes (FLS) stimulated with lipopolysaccharide (LPS; 10 μg/ml) for 24 hr (lane 1) or unstimulated (lane 2). Results are representative data obtained with cells isolated from one RA patients out of the three tested. (b) Expression of ASC and NALP3 by Western blot. The RA FLS cellular extracts, stimulated with LPS (10 μg/ml) for 24 hr (lane 1) or unstimulated (lane 2) were analysed by Western blot with anti-ASC or anti-NALP3 antibodies. (c) Expression of ASC and NALP3 by immunohistochemistry. Unstimulated RA FLS were stained with anti-ASC or anti-NALP3 antibodies. A negative control section (control) incubated with pre-immune serum is shown at the bottom. Original magnification × 400.