Figure 8.

Tic62 Has a Stabilizing Effect on FNR in Vitro.

Catalytic activity of overexpressed and purified FNR was measured in vitro using the Fd-dependent Cyt c reduction assay. FNR was either used fresh (white), or activity was determined after overnight incubation (black). Both values are additionally represented by dotted lines for comparison with the other results. Various amounts (molar ratio of FNR:used protein was 1:2, 1:1, and 1:0.5) of full-length Tic62 (light gray), Tic62 C terminus (gray), or egg albumin as control (dark gray) were added to FNR before overnight incubation, and activity was measured the next day. None of the interacting proteins displayed any detectable Cyt c reductase activity independently of FNR. The mean values of triplicate experiments with se bars are depicted.