Figure 4.

Protein Blot Analysis and Effect of Chaotrope Treatment on Enzyme Activities in Tonoplast of M. crystallinum.

(A) Coomassie blue–stained gel of tonoplast isolated from control (C) and salt (S)-treated plants (200 mM NaCl for 1 week) incubated in the presence (+) or absence (−) of 200 mM Na₂CO₃, pH 11.4, and 3 mM MgATP. Proteins that are clearly absent in the chaotrope treated lanes are marked (asterisks).

(B) Protein blot analysis of tonoplast from salt-treated plants incubated in the presence (+) or absence (−) of 200 mM Na₂CO₃, pH 11.4, and 3 mM MgATP and probed with antibodies against V-ATPase subunits VHA-B, VHA-E, and VHA-A and the enzymes aldolase and enolase.

(C) Aldolase activity in tonoplast from salt-treated plants incubated in the presence (+ chaotrope) or absence (− chaotrope) of 200 mM Na₂CO₃, pH 11.4, and 3 mM MgATP. Values are means ± se from three experiments.

(D) V-ATPase hydrolytic activity in tonoplast from salt-treated plants incubated in the presence (+ chaotrope) or absence (− chaotrope) of 200 mM Na₂CO₃, pH 11.4, and 3 mM MgATP. Values are means ± se from three experiments.