Figure 5.

Aldolase Interacts with the VHA-B Subunit of the V-ATPase.

Leaf tonoplast protein (15 μg) from control (C) and salt-treated (S) M. crystallinum plants was analyzed by reciprocal immunoprecipitation (IP), SDS-PAGE, and immunoblotting (IB) as described in Methods, using the indicated antibodies (top antibody was used for immunoprecipitation; bottom antibody was used to probe blots). The results shown are representative of experiments that were repeated three times, which yielded identical results. The positions of PAGE molecular mass markers are shown in kilodaltons on the left of the panels.