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. 2009 Dec;21(12):4002–4017. doi: 10.1105/tpc.109.071282

Figure 1.

Figure 1.

Effect of Am GPPS.SSU Expression on the Phenotype of Transgenic Tobacco Plants.

(A) Am GPPS.SSU protein levels in different transgenic tobacco lines. The representative protein gel blot shows the 30-kD protein recognized by anti-Am GPPS.SSU antibodies. Proteins were extracted from tobacco leaves of untransformed control and seven independent transgenic lines (shown on the top), and 20 μg of protein were loaded in each lane. The blot shown represents a typical result of three independent experiments.

(B) Effect of high levels of Am GPPS.SSU protein on the phenotype of transgenic tobacco plants. Three transgenic lines with the highest levels of Am GPPS.SSU protein (GPPS.SSU-5 to GPPS.SSU-7) show strong chlorosis and dwarfism relative to the control plant shown in (C).

(C) Phenotype of GPPS.SSU-1 line, which in contrast with GPPS.SSU-5 to GPPS.SSU-7, was able to produce mature plants. The inset shows leaves from control (left) and GPPS.SSU-1 transgenic plants (right). All plants in (B) and (C) are 6 to 7 weeks old.