Figure 3.

Effect of Am GPPS.SSU Expression on GGPP-Derived Isoprenoids in Transgenic Tobacco Plants.

(A) Chlorophyll and carotenoid contents in the leaves of control and transgenic lines GPPS.SSU-1 and GPPS.SSU-3. Data are means ± sd (n = 3 to 10). Asterisks indicate values that are significantly different from the control. Confidence levels were tested by a Student's t test; * P < 0.05; ** P < 0.001.

(B) Levels of GAs in the leaves of control and transgenic lines GPPS.SSU-1 and GPPS.SSU-3.

(C) Germination of T1 seeds of GPPS.SSU-1 line without (top panel) or with (bottom panel) 30 μM GA₃. Resulting 10-d-old T1 seedlings show the expected segregation of the wild type and bleached/reduced growth phenotype. Addition of GA₃ rescued the growth of transgenic seedlings.

(D) Continued treatment of transgenic GPPS.SSU-1 seedlings with GA₃ recovered their growth to almost that of control plants. Fourteen-day-old plants grown without (top panel) or with (bottom panel) 30 μM GA₃ are shown.