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. 2009 Dec;21(12):3868–3884. doi: 10.1105/tpc.109.068700

Figure 3.

Figure 3.

AFH3 FH1FH2 Binds to Barbed Ends and Blocks Actin Polymerization and Depolymerization from Barbed Ends.

(A) Kinetics of actin filament barbed-end elongation in the presence of AFH3 FH1FH2. Preformed actin filaments (0.8 μM) were incubated with various concentrations of AFH3 FH1FH2 before the addition of 1 μM pyrene-actin monomers. The concentration of AFH3 FH1FH2 from top to the bottom: 0, 300, 450, 600, 1200, and 2000 nM.

(B) Variation in the initial rate of elongation as a function of AFH3 FH1FH2 concentration. The data were fit with Equation 1 (see Methods) to determine the equilibrium dissociation constant value of 307 nM for AFH3 FH1FH2.

(C) Kinetics of actin depolymerization in the presence of various concentrations of AFH3 FH1FH2. AFH3 FH1FH2 was incubated with 5 μM F-actin for 5 min before dilution of the solution 25-fold into Buffer G. The concentration of AFH3 FH1FH2 from bottom to top: 0, 50, 70, 150, 200, and 400 nM.