Figure 4.

AFH3 FH1FH2 Inhibits Reannealing of Actin Filaments.

(A) to (I) Four micromolar polymerized actin was stabilized with 4 μM rhodamine-phalloidin, sheared by a needle, and then allowed to reanneal in the presence of buffer, 300 nM AFH1 FH1FH2, or 300 nM AFH3 FH1FH2. Samples were diluted at the indicated time points and visualized by fluorescence microscopy. Bar in (A) = 10 μm and applies to (A) to (I).

(J) Mean length of actin filaments (±se) at different time points. Actin alone, black bars; Actin plus AFH1 FH1FH2, white bars; Actin plus AFH3 FH1FH2, crosshatched bars.