Figure 7.

Cytoplasmic Streaming Direction Was Altered and the Velocity of Cytoplasmic Streaming Was Reduced in AFH3 RNAi Pollen Tubes.

(A) Schematic representation of the cytoplasmic streaming pattern in wild-type ([a] and [b]) and AFH3 RNAi ([c] to [e]) pollen tubes. Red arrows represent the direction of cytoplasmic streaming that moves particles toward the tip of the pollen tube; green arrows represent the direction of cytoplasmic streaming that moves particles backward in the pollen tube; the black lines in the pollen tube indicate the cytoplasmic streaming track.

(B) The velocity of cytoplasmic streaming was analyzed by Image J software (http://rsbweb.nih.gov/ij/). Twenty cytoplasmic streaming particles from several independent pollen tubes were measured for each genotype. and the mean values (±se) are plotted.