Figure 5.

The Effect of Ethylene, ACC, and ABA on the Time Course of Endosperm Rupture and on the Germination Rates GR_X% of L. sativum FR14 Seeds.

(A) and (B) The times to reach 15 or 50% endosperm rupture (t_15% or t_50%) of the seed population were determined from the time courses of endosperm rupture in medium without (CON) and with additions (NBD, ABA, ACC, C₂H₄, or combinations). Germination rates GR_15% or GR_50% were then calculated (GR_X% = 1/t_X%) and used in subsequent analyses (gray arrows). The effect of ACC on time course (A) and GR_15% (B) of seeds treated without (CON) or with NBD or ABA added.

(C) The effect of C₂H₄ on GR_15% and GR_50% of seeds imbibed without (CON) or with NBD or ABA. Numbers above the columns are fold increases in GR_X% from the addition of ACC or C₂H₄ (GR_X% ratios ±ACC or ±C₂H₄). Medium additions, as indicated: 5 μM ABA, 1 mM ACC, 100 μL/L NBD, and 70 μL/L ethylene. Mean values ± se of 3 × 50 seeds are presented.