Figure 8.

The Effect of ACC, NBD, or NBD+ACC on Endogenous ABA Contents and ACO2 Transcript Expression during Germination of L. sativum FR14.

(A) Endogenous ABA concentrations of whole seeds incubated without (CON) or with 1 mM ACC or 100 μL/L NBD or ACC+NBD added to the medium in continuous light at 18°C. FW, fresh weight.

(B) Lepidium ACO2 transcript expression pattern determined by qRT-PCR in whole seeds. Relative ΔΔC_t expression values based on the comparison with validated constitutive transcripts are presented.

Mean values ± se (A) or +se (B) are presented for three biologically independent samples each with 50 seeds.